Modification:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Cell Isolation:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Lysis:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Enzyme-linked Immunosorbent Assay:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Recombinant:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Isolation:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
Multiplex Assay:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
AST Assay:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
RNA Sequencing:Article Title: Synergically enhanced anti-tumor immunity of in vivo panCAR by circRNA vaccine boosting
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER BRITELITE PLUS Perkinelmer Cat#6066761 DMEM (Dulbecco’s Modified Eagle’s Medium) Coring Cat#10-013-CV Fetal Bovine Serum Biological Industries Cat#C04001-500 Bovine Serum Albumin Merck Cat#B2064 RPMI 1640 BasalMedia Cat#L220KJ Pme I New England Biolabs Cat#R0560L DNase I New England Biolabs Cat#M0303L RNase R Epicentre Cat#RNR07250 Quick CIP New England Biolabs Cat#M0525L RNase Inhibitor, Murine New England Biolabs Cat#M0314L m7G(5′)ppp(5′)G RNA Cap Structure Analog New England Biolabs Cat#S1404S C12-200 AVT Cat#CK0239 Percoll cell isolation solution GE (Pharmacia) Cat#17089102 ACK Lysis Buffer Solarbio Cat#R1010 DOPE AVT Cat#S03005 ELISA Coating Solution bioss Cat#C04-01001 ELISA Stop Solution bioss Cat#C04-01003 ELISA Washing Buffer bioss Cat#C04-01004 1-Step TMB ELISA Substrate Solutions Thermo Cat#34028 BSA Sigma Cat#V900933 Recombinant human IL-2 protein MCE Cat#HY-P70758 HER2/CD340 Protein, Human MCE Cat#HY-P70254 Critical commercial assays DNA Clean & Concentrator Kit Zymo Research Cat#D4034 T7 High Yield RNA Synthesis Kit New England Biolabs Cat#E2040S RNA Clean & Concentrator Kit Zymo Research Cat#R1017 Monarch RNA Cleanup Kit New England Biolabs Cat#T2040L Quant-it RiboGreen RNA Assay Kit Thermo Fisher Scientific Cat#R11490 NEBNext Ultra II RNA Library Prep Kit for Illumina New England Biolabs CatE7770S NEBNext Poly(A) mRNA Magnetic Isolation Module New England Biolabs Cat#E7490L NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) New England Biolabs Cat#E7335S Mouse IL-6 ELISA Kit ABclonal Cat#RK00008 Mouse IL-1β ELISA Kit ABclonal Cat#RK00006 Mouse TNF-α ELISA Kit ABclonal Cat#RK00027 Mouse ALT ELISA Kit ABclonal Cat#RK06383 Mouse AST ELISA Kit ABclonal Cat#RK08442 Mouse ALP ELISA Kit ABclonal Cat#RK02586 Agencourt AMPure XP Kit Beckman Cat#A63881 Deposited data RNA-seq This paper GEO: GSE268105 Experimental models: Cell lines HEK293T This paper N/A RAW 264.7 Shanghai Zhong Qiao Xin Zhou Biotechnology Co., Ltd. Cat#ZQ0098 4T1-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0066-022SS B16F10-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0106-010SS CT26-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0063-019SS MC38-HER2-Luc-EGFP Vigen Biotechnology Co., Ltd. Cat#VGC-0062-031SS (Continued on next page) Cell Reports Medicine 6, 102250, August 19, 2025 e2 Article ll OPEN ACCESS
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